The Meltzer Lab uses spatial transcriptomics, a technique that maps gene activity while preserving a cell’s location, to create a high-resolution atlas of the developing human cerebral cortex. Our results revealed distinct spatial and laminar distributions of neuronal subtypes, including axial level and sex-specific differences. We also identified several neuronal subtypes with altered transcriptomic and predicted cell-cell communications in a model of neuropathic pain. Our spatially resolved cellular and molecular map of the spinal cord can facilitate the decoding of circuit mechanisms underlying somatosensory and motor functions in health and disease.

Wildtype 6 runs:

1. https://webatlas.cog.sanger.ac.uk/latest/index.html?config=https://qwneuron.github.io/MERFISH-mouse-spinal-cord-atlas/0328-wildtype-config.json

2. https://webatlas.cog.sanger.ac.uk/latest/index.html?config=https://qwneuron.github.io/MERFISH-mouse-spinal-cord-atlas/0415-wildtype-config.json

3. https://webatlas.cog.sanger.ac.uk/latest/index.html?config=https://qwneuron.github.io/MERFISH-mouse-spinal-cord-atlas/0422-wildtype-config.json

4. https://webatlas.cog.sanger.ac.uk/latest/index.html?config=https://qwneuron.github.io/MERFISH-mouse-spinal-cord-atlas/0503-wildtype-config.json

5. https://webatlas.cog.sanger.ac.uk/latest/index.html?config=https://qwneuron.github.io/MERFISH-mouse-spinal-cord-atlas/0610-wildtype-config.json

6. https://webatlas.cog.sanger.ac.uk/latest/index.html?config=https://qwneuron.github.io/MERFISH-mouse-spinal-cord-atlas/0613-wildtype-config.json

SNI 3 runs:

1. https://webatlas.cog.sanger.ac.uk/latest/index.html?config=https://qwneuron.github.io/MERFISH-mouse-spinal-cord-atlas/0717-SNI-config.json

2. https://webatlas.cog.sanger.ac.uk/latest/index.html?config=https://qwneuron.github.io/MERFISH-mouse-spinal-cord-atlas/0722-SNI-config.json

3. https://webatlas.cog.sanger.ac.uk/latest/index.html?config=https://qwneuron.github.io/MERFISH-mouse-spinal-cord-atlas/0825-SNI-config.json